SM3430 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 0 Phosphorothioate* Phosphodiester
siRNA modification types 0 2
Overall number of modifications 0 11
Position of modifications 0 3,9,12,17,18,19 * 3,9,12,17,18
Modification on sugar or base or phosphate 0 Sugar
SMILES
(Click to view structure & nomenclature)
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OCC1OCC(O)C1OP(O)(S)=O
OCC1OCC(O)C1OP(O)([O-])=O


siRNA Sequence GGAGAUCAACAUUUUCAAA UUUGAAAAUGUUGAUCUCC
siRNA length base-pair 19 19
siRNA name in paper 11 10
Biological activity IC-50 = 5580 nM for target mRNA
Experiment used to check activity Real-time PCR
Melting temperature (oC) NA
Target gene Survivin gene
siRNA concentration 200 nM
Cell or Organism used U-87 MG cells
Transfection method Lipofectin (Invitrogen)
Duration after transfection 16 Hours
Article title POSITIONALLY MODIFIED siRNA CONSTRUCTS
Reference US8394947, EP1765074B1