Field | Sense | Antisense |
siRNA chemical modification | 0 | Phosphorothioate* Phosphodiester* 2-O-Methyl |
siRNA modification types | 0 | 3 |
Overall number of modifications | 0 | 15 |
Position of modifications | 0 | 1,2,3,9,12 * 1,2,3,9,12,17,18 * 17,18,19 |
Modification on sugar or base or phosphate | 0 | Sugar |
SMILES (Click to view structure & nomenclature) |
- |
OCC1OCC(O)C1OP(O)(S)=O OCC1OCC(O)C1OP(O)([O-])=O COC1C(O)OC(CO)C1O |
siRNA Sequence | GGAGAUCAACAUUUUCAAA | UUUGAAAAUGUUGAUCUCC |
siRNA length base-pair | 19 | 19 |
siRNA name in paper | 11 | 10 |
Biological activity | IC-50 = 132.16 nM for target mRNA | |
Experiment used to check activity | Real-time PCR | |
Melting temperature (oC) | NA | |
Target gene | Survivin gene | |
siRNA concentration | 200 nM | |
Cell or Organism used | HeLa cells | |
Transfection method | Lipofectin (Invitrogen) | |
Duration after transfection | 16 Hours | |
Article title | POSITIONALLY MODIFIED siRNA CONSTRUCTS |
|
Reference | US8394947, EP1765074B1 |