SM3445 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 2-O-Methyl 2-O-Methyl
siRNA modification types 1 1
Overall number of modifications 2 1
Position of modifications 7,8 15
Modification on sugar or base or phosphate Sugar Sugar
SMILES
(Click to view structure & nomenclature)
COC1C(O)OC(CO)C1O



COC1C(O)OC(CO)C1O



siRNA Sequence AGCGCUUAAAGGGCGAGAAGAUU UCUUCUCGCCCUUUAAGCGCUUU
siRNA length base-pair 23 23
siRNA name in paper 1-3C 1-3C
Biological activity 56 percent target mRNA inhibition
Experiment used to check activity Dual luciferase reporter assay
Melting temperature (oC) NA
Target gene Human KAZRIN gene
siRNA concentration 13 nM
Cell or Organism used HEK293 cells
Transfection method Lipofectamine 2000
Duration after transfection 4 Hours
Article title Modified oligo-nucleic acid molecule, preparation method and uses thereof 
Reference US20120088815A1, EP2415869A1