Field | Sense | Antisense |
siRNA chemical modification | 2-Deoxy-2-Fluoro | 2-Deoxy-2-Fluoro |
siRNA modification types | 1 | 1 |
Overall number of modifications | 4 | 9 |
Position of modifications | 2,3,8,9 | 5,7,13,14,15,19,20,21,23 |
Modification on sugar or base or phosphate | Sugar | Sugar |
SMILES (Click to view structure & nomenclature) |
OCC1OCC(F)C1O |
OCC1OCC(F)C1O |
siRNA Sequence | UUAAUGCUAAUCGUGAUAGGGGUU | CCCCUAUCACGAUUAGCAUUAAUA |
siRNA length base-pair | 24 | 24 |
siRNA name in paper | 3-3M | 3-3M |
Biological activity | 28 percent target mRNA inhibition | |
Experiment used to check activity | Dual luciferase reporter assay | |
Melting temperature (oC) | NA | |
Target gene | Human BIC gene | |
siRNA concentration | 13 nM | |
Cell or Organism used | HEK293 cells | |
Transfection method | Lipofectamine 2000 | |
Duration after transfection | 4 Hours | |
Article title | Modified oligo-nucleic acid molecule, preparation method and uses thereof |
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Reference | US20120088815A1, EP2415869A1 |