Field | Sense | Antisense |
siRNA chemical modification | 2-O-Methyl | 2-O-Methyl |
siRNA modification types | 1 | 1 |
Overall number of modifications | 10 | 2 |
Position of modifications | 2,4,6,8,10,12,14,16,18,20 | 7,18 |
Modification on sugar or base or phosphate | Sugar | Sugar |
SMILES (Click to view structure & nomenclature) |
COC1C(O)OC(CO)C1O |
COC1C(O)OC(CO)C1O |
siRNA Sequence | CACAGAAAGAGUUCGAGGAGTT | CUCCUCGAACUCUUUCUGUTT |
siRNA length base-pair | 22 | 22 |
siRNA name in paper | 168-2 | 168-2 |
Biological activity | 37 percent target mRNA inhibition | |
Experiment used to check activity | Dual luciferase reporter assay | |
Melting temperature (oC) | NA | |
Target gene | BIRC5 gene | |
siRNA concentration | 13 nM | |
Cell or Organism used | HEK293 cells | |
Transfection method | Lipofectamine 2000 | |
Duration after transfection | 4 Hours | |
Article title | Modified oligo-nucleic acid molecule, preparation method and uses thereof |
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Reference | US20120088815A1, EP2415869A1 |