| Field | Sense | Antisense |
| siRNA chemical modification | L-isonucleoside thymidine* 2-Deoxythymidine | 2-Deoxythymidine |
| siRNA modification types | 2 | 1 |
| Overall number of modifications | 3 | 2 |
| Position of modifications | 1 * 20,21 | 20,21 |
| Modification on sugar or base or phosphate | Sugar ; Sugar | Sugar |
| SMILES (Click to view structure & nomenclature) |
CC1=CN(C2COC(CO)C2OP(O)([O-])=O)C(=O)NC1=O CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O |
OCC1OCCC1O |
| siRNA Sequence | UCGGGAAAUUUCUCUAUUATT | UAAUAGAGAAAUUUCCCGATT |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | AI | B |
| Biological activity | 13 percent target mRNA inhibition | |
| Experiment used to check activity | Dual luciferase assay | |
| Melting temperature (oC) | NA | |
| Target gene | Cdc-gene strand A | |
| siRNA concentration | 13 nM | |
| Cell or Organism used | HEK293 cells | |
| Transfection method | Lipofectamine 2000 | |
| Duration after transfection | 24 Hours | |
| Article title | Synthesis and properties of novel L-isonucleoside modified oligonucleotides and siRNAs |
|
| Reference | 22895883 | |