SM4229 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 2-Deoxythymidine L-isonucleoside adenosine* 2-Deoxythymidine
siRNA modification types 1 2
Overall number of modifications 2 3
Position of modifications 20,21 19 * 20,21
Modification on sugar or base or phosphate Sugar Sugar ; Sugar
SMILES
(Click to view structure & nomenclature) 		CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O
NC1=C2N=CN(C3COC(CO)C3OP(O)([O-])=O)C2=NC=N1
CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O
siRNA Sequence UCGGGAAAUUUCUCUAUUATT UAAUAGAGAAAUUUCCCGATT
siRNA length base-pair 21 21
siRNA name in paper A BII
Biological activity 90 percent target mRNA inhibition
Experiment used to check activity Dual luciferase assay
Melting temperature (oC) NA
Target gene Cdc-gene strand A
siRNA concentration 13 nM
Cell or Organism used HEK293 cells
Transfection method Lipofectamine 2000
Duration after transfection 24 Hours
Article title Synthesis and properties of novel L-isonucleoside modified oligonucleotides and siRNAs
Reference22895883