SM4244 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification Serinol nucleic acid Serinol nucleic acid
siRNA modification types 1 1
Overall number of modifications 2 4
Position of modifications 1,2 1,2,22,23
Modification on sugar or base or phosphate Sugar Sugar
SMILES
(Click to view structure & nomenclature)
CC(=O)NC(CO)COP(O)(O)=O



CC(=O)NC(CO)COP(O)(O)=O



siRNA Sequence AACCUUACCCACCUCAUGUAUCU AGAUACAUGAGGUGGGUAAGGUU
siRNA length base-pair 23 23
siRNA name in paper S5 A53
Biological activity 76 percent target mRNA inhibition
Experiment used to check activity Dual luciferase assay
Melting temperature (oC) NA
Target gene Firefly luciferase expression
siRNA concentration 17 nM
Cell or Organism used 293FT cells
Transfection method Lipofectamine 2000
Duration after transfection 48 Hours
Article title Enhancement of stability and activity of siRNA by terminal substitution with serinol nucleic acid (SNA)
Reference25233814