Field | Sense | Antisense |
siRNA chemical modification | 0 | 2-O-Methyl ribose |
siRNA modification types | 0 | 1 |
Overall number of modifications | 0 | 4 |
Position of modifications | 0 | 1,2,22,23 |
Modification on sugar or base or phosphate | 0 | Sugar |
SMILES (Click to view structure & nomenclature) |
- |
COC1C(O)OC(CO)C1O |
siRNA Sequence | AACCUUACCCACCUCAUGUAUCU | AGAUACAUGAGGUGGGUAAGGUU |
siRNA length base-pair | 23 | 23 |
siRNA name in paper | S | OMe-A53 |
Biological activity | 62 percent target mRNA inhibition | |
Experiment used to check activity | Dual luciferase assay | |
Melting temperature (oC) | NA | |
Target gene | Firefly luciferase expression | |
siRNA concentration | 17 nM | |
Cell or Organism used | 293FT cells | |
Transfection method | Lipofectamine 2000 | |
Duration after transfection | 48 Hours | |
Article title | Enhancement of stability and activity of siRNA by terminal substitution with serinol nucleic acid (SNA) |
|
Reference | 25233814 |