SM4292 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification Serinol nucleic acid Phosphate* Serinol nucleic acid
siRNA modification types 1 2
Overall number of modifications 4 3
Position of modifications 1,2,22,23 1 * 20,21
Modification on sugar or base or phosphate Sugar Sugar
SMILES
(Click to view structure & nomenclature)
CC(=O)NC(CO)COP(O)(O)=O



OCC1OCC(O)C1OP(O)([O-])=O
CC(=O)NC(CO)COP(O)(O)=O


siRNA Sequence AACCUUACCCACCUCAUGUAUCU AUACAUGAGGUGGGUAAGGUU
siRNA length base-pair 23 21
siRNA name in paper S53 21A3-5P
Biological activity 78 percent target mRNA inhibition
Experiment used to check activity Dual luciferase assay
Melting temperature (oC) NA
Target gene Firefly luciferase expression
siRNA concentration 17 nM
Cell or Organism used 293FT cells
Transfection method Lipofectamine 2000
Duration after transfection 48 Hours
Article title Enhancement of stability and activity of siRNA by terminal substitution with serinol nucleic acid (SNA)
Reference25233814