Field | Sense | Antisense |
siRNA chemical modification | 0 | Phosphorothioate* 2-O-Methylribose |
siRNA modification types | 0 | 2 |
Overall number of modifications | 0 | 23 |
Position of modifications | 0 | 1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20 * 6,7,8 |
Modification on sugar or base or phosphate | 0 | Sugar ; Phosphate |
SMILES (Click to view structure & nomenclature) |
- |
OCC1OCC(O)C1OP(O)(S)=O COC1C(O)OC(CO)C1O |
siRNA Sequence | AAGUAAGGACCAGAGACAAA | UUUGUCUCUGGUCCUUACUU |
siRNA length base-pair | 20 | 20 |
siRNA name in paper | 2 | 13 |
Biological activity | 69 percent target mRNA inhibition | |
Experiment used to check activity | Real-time PCR | |
Melting temperature (oC) | NA | |
Target gene | PTEN mRNA | |
siRNA concentration | 1.5 nM | |
Cell or Organism used | HeLa cells | |
Transfection method | Lipofectin | |
Duration after transfection | 20 Hours | |
Article title | Positional effect of chemical modifications on short interference RNA activity in mammalian cells |
|
Reference | 15974578 |