| Field | Sense | Antisense |
| siRNA chemical modification | 2-O-(2-methoxyethyl)ribose | Phosphorothioate |
| siRNA modification types | 1 | 1 |
| Overall number of modifications | 3 | 20 |
| Position of modifications | 1,2,3 | 1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20 |
| Modification on sugar or base or phosphate | Sugar | Phosphate |
| SMILES (Click to view structure & nomenclature) |
COCCOC1COC(CO)C1O |
OCC1OCC(O)C1OP(O)(S)=O |
| siRNA Sequence | AAGUAAGGACCAGAGACAAA | UUUGUCUCUGGUCCUUACUU |
| siRNA length base-pair | 20 | 20 |
| siRNA name in paper | 32 | 3 |
| Biological activity | 56 percent target mRNA inhibition | |
| Experiment used to check activity | Real-time PCR | |
| Melting temperature (oC) | NA | |
| Target gene | PTEN mRNA | |
| siRNA concentration | 2 nM | |
| Cell or Organism used | HeLa cells | |
| Transfection method | Lipofectin | |
| Duration after transfection | 20 Hours | |
| Article title | Positional effect of chemical modifications on short interference RNA activity in mammalian cells |
|
| Reference | 15974578 | |