SM4390 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification Triazole-linked nucleic acid* Phenyl* 2-Deoxythymidine 2-Deoxythymidine
siRNA modification types 3 1
Overall number of modifications 4 2
Position of modifications 7 * 8 * 20,21 20,21
Modification on sugar or base or phosphate Phosphate ; Base ; Sugar Sugar
SMILES
(Click to view structure & nomenclature) 		CCN(CCN1C=C(CN(CC)C(C)=O)N=N1)C(C)=O
OP(O)(=O)OCCCCCCNC(=O)COC1=CC=CC=C1
CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O
CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O
siRNA Sequence CUUACGCUGAGUACUUCGATT UCGAAGUACUCAGCGUAAGTT
siRNA length base-pair 21 21
siRNA name in paper aL4 aL4
Biological activity 95 percent target mRNA inhibition
Experiment used to check activity Dual luciferase reporter assay
Melting temperature (oC) 59.2
Target gene Firefly luciferase gene
siRNA concentration 0.08 nM
Cell or Organism used HeLa cells
Transfection method Lipofectamine 2000
Duration after transfection 48 Hours
Article title Conjugation and Evaluation of Small Hydrophobic Molecules to Triazole-Linked siRNAs
Reference25699137