| Field | Sense | Antisense |
| siRNA chemical modification | 2-Deoxythymidine* Cholesterol | 2-Deoxythymidine |
| siRNA modification types | 2 | 1 |
| Overall number of modifications | 3 | 2 |
| Position of modifications | 1 * 20,21 | 20,21 |
| Modification on sugar or base or phosphate | Sugar ; Base | Sugar |
| SMILES (Click to view structure & nomenclature) |
CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O CC(C)CCCC(C)C1CCC2C3CC=C4CC(O)CCC4(C)C3CCC12C |
CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O |
| siRNA Sequence | CUUACGCUGAGUACUUCGATT | UCGAAGUACUCAGCGUAAGTT |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | aL14 | aL14 |
| Biological activity | 90 percent target mRNA inhibition | |
| Experiment used to check activity | Dual luciferase reporter assay | |
| Melting temperature (oC) | 65.3 | |
| Target gene | Firefly luciferase gene | |
| siRNA concentration | 0.08 nM | |
| Cell or Organism used | HeLa cells | |
| Transfection method | Lipofectamine 2000 | |
| Duration after transfection | 48 Hours | |
| Article title | Conjugation and Evaluation of Small Hydrophobic Molecules to Triazole-Linked siRNAs |
|
| Reference | 25699137 | |