| Field | Sense | Antisense |
| siRNA chemical modification | Triazole-linked nucleic acid* Phenyl* 2-Deoxythymidine | 2-Deoxythymidine |
| siRNA modification types | 3 | 1 |
| Overall number of modifications | 4 | 2 |
| Position of modifications | 16 * 17 * 20,21 | 20,21 |
| Modification on sugar or base or phosphate | Phosphate ; Base ; Sugar | Sugar |
| SMILES (Click to view structure & nomenclature) |
CCN(CCN1C=C(CN(CC)C(C)=O)N=N1)C(C)=O OP(O)(=O)OCCCCCCNC(=O)COC1=CC=CC=C1 CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O |
CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O |
| siRNA Sequence | GUGAAGUCAACAUGCCUGCTT | GCAGGCAUGUUGACUUCACTT |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | aB1 | aB1 |
| Biological activity | 83 percent target mRNA inhibition | |
| Experiment used to check activity | Dual luciferase reporter assay | |
| Melting temperature (oC) | NA | |
| Target gene | Anti-bcl-2 (aB) gene | |
| siRNA concentration | 10 nM | |
| Cell or Organism used | HeLa cells | |
| Transfection method | Lipofectamine 2000 | |
| Duration after transfection | 48 Hours | |
| Article title | Conjugation and Evaluation of Small Hydrophobic Molecules to Triazole-Linked siRNAs |
|
| Reference | 25699137 | |