SM4428 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification [1,1-Biphenyl]-3,5-dimethanol [1,1-Biphenyl]-3,5-dimethanol
siRNA modification types 1 1
Overall number of modifications 2 2
Position of modifications 20,21 20,21
Modification on sugar or base or phosphate Base Base
SMILES
(Click to view structure & nomenclature) 		OCC1=CC(=CC(CO)=C1)C1=CC=CC=C1
OCC1=CC(=CC(CO)=C1)C1=CC=CC=C1
siRNA Sequence GGCCUUUCACUACUCCUACTT GUAGGAGUAGUGAAAGGCCTT
siRNA length base-pair 21 21
siRNA name in paper siRNA19 siRNA19
Biological activity 43 percent target mRNA inhibition
Experiment used to check activity Dual luciferase reporter assay
Melting temperature (oC) 79.9
Target gene Renilla luciferase
siRNA concentration 1 nM
Cell or Organism used HeLa cells
Transfection method Lipofectamine RNAiMAX (Invitrogen) and Opti-MEM (GIBCO-BRL)
Duration after transfection 24 Hours
Article title Incorporation of biaryl units into the 5' and 3' ends of sense and antisense strands of siRNA duplexes improves strand selectivity and nuclease resistance
Reference21141919