| Field | Sense | Antisense |
| siRNA chemical modification | [3-(hydroxymethyl)-5-naphthalen-1-ylphenyl]methanol* [3-(hydroxymethyl)phenyl]methanol | [3-(hydroxymethyl)-5-naphthalen-1-ylphenyl]methanol* 1,3-Benzenedimethanol |
| siRNA modification types | 2 | 2 |
| Overall number of modifications | 3 | 2 |
| Position of modifications | 1 * 21,22 | 20 * 21 |
| Modification on sugar or base or phosphate | Base | Base |
| SMILES (Click to view structure & nomenclature) |
OCC1=CC(=CC(CO)=C1)C1=CC=CC2=CC=CC=C12 OCC1=CC(CO)=CC=C1 |
OCC1=CC(=CC(CO)=C1)C1=CC=CC2=CC=CC=C12 OCC1=CC(CO)=CC=C1 |
| siRNA Sequence | UAAGAUGUUCAUCGAGUCCTT | GGACUCGAUGAACAUCUUATT |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | siRNA35 | siRNA35 |
| Biological activity | 58 percent target mRNA inhibition | |
| Experiment used to check activity | Dual luciferase reporter assay | |
| Melting temperature (oC) | NA | |
| Target gene | Renilla luciferase | |
| siRNA concentration | 1 nM | |
| Cell or Organism used | HeLa cells | |
| Transfection method | Lipofectamine RNAiMAX (Invitrogen) and Opti-MEM (GIBCO-BRL) | |
| Duration after transfection | 24 Hours | |
| Article title | Incorporation of biaryl units into the 5' and 3' ends of sense and antisense strands of siRNA duplexes improves strand selectivity and nuclease resistance |
|
| Reference | 21141919 | |