| Field | Sense | Antisense |
| siRNA chemical modification | [3-(hydroxymethyl)-5-naphthalen-1-ylphenyl]methanol* 2-Deoxyribonucleotide* [3-(hydroxymethyl)phenyl]methanol | 2-Deoxyribonucleotide* [3-(hydroxymethyl)-5-naphthalen-1-ylphenyl]methanol* [3-(hydroxymethyl)phenyl]methanol |
| siRNA modification types | 3 | 3 |
| Overall number of modifications | 7 | 6 |
| Position of modifications | 1 * 7,11,14,18 * 22,23 | 4,8,11,15 * 21 * 22 |
| Modification on sugar or base or phosphate | Base | Base |
| SMILES (Click to view structure & nomenclature) |
OCC1=CC(=CC(CO)=C1)C1=CC=CC2=CC=CC=C12 OCC1OCCC1O OCC1=CC(CO)=CC=C1 |
OCC1OCCC1O OCC1=CC(=CC(CO)=C1)C1=CC=CC2=CC=CC=C12 OCC1=CC(CO)=CC=C1 |
| siRNA Sequence | GUCUCGUAGACCGUGCAUCATT | UGAUGCACGGUCUACGAGACTT |
| siRNA length base-pair | 22 | 22 |
| siRNA name in paper | siRNA42 | siRNA42 |
| Biological activity | NA | |
| Experiment used to check activity | NA | |
| Melting temperature (oC) | NA | |
| Target gene | Renilla luciferase | |
| siRNA concentration | 1 nM | |
| Cell or Organism used | HeLa cells | |
| Transfection method | Lipofectamine RNAiMAX (Invitrogen) and Opti-MEM (GIBCO-BRL) | |
| Duration after transfection | 24 Hours | |
| Article title | Incorporation of biaryl units into the 5' and 3' ends of sense and antisense strands of siRNA duplexes improves strand selectivity and nuclease resistance |
|
| Reference | 21141919 | |