Field | Sense | Antisense |
siRNA chemical modification | 0 | Palmitic Acid |
siRNA modification types | 0 | 1 |
Overall number of modifications | 0 | 1 |
Position of modifications | 0 | 21 |
Modification on sugar or base or phosphate | 0 | Phosphate |
SMILES (Click to view structure & nomenclature) |
- |
CCCCCCCCCCCCCCCC(=O)NCCCCCCO |
siRNA Sequence | GGCCUUUCACUACUCCUACGA | GUAGGAGUAGUGAAAGGCCAG |
siRNA length base-pair | 21 | 21 |
siRNA name in paper | siLuc- C16 | siLuc- C16 |
Biological activity | 82 percent target mRNA inhibition | |
Experiment used to check activity | Real-time PCR | |
Melting temperature (oC) | NA | |
Target gene | Renilla luciferase | |
siRNA concentration | 1 nM | |
Cell or Organism used | HeLa cells | |
Transfection method | Lipofectamine 2000 | |
Duration after transfection | 48 Hours | |
Article title | Palmitic acid-conjugated 21-nucleotide siRNA enhances gene-silencing activity |
|
Reference | 21985606 |