SM4464 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 2-Deoxyribonucleotide* Inverted thymidine 2-Deoxyribonucleotide* Inverted thymidine
siRNA modification types 2 2
Overall number of modifications 2 2
Position of modifications 20 * 21 20 * 21
Modification on sugar or base or phosphate Sugar Sugar
SMILES
(Click to view structure & nomenclature) 		OCC1OCCC1O
CC1=CN(C2CC(OP([O-])(=O)OC3C(O)COC3CO)C(CO)O2)C(=O)NC1=O
OCC1OCCC1O
CC1=CN(C2CC(OP([O-])(=O)OC3C(O)COC3CO)C(CO)O2)C(=O)NC1=O
siRNA Sequence UCCUACAGCACAACAAAUGUG CAUUUGUUGUGCUGUAGGATT
siRNA length base-pair 21 21
siRNA name in paper siVEGF-idT siVEGF-idT
Biological activity 65 percent target mRNA inhibition
Experiment used to check activity Real-time PCR
Melting temperature (oC) NA
Target gene vascular endothelial growth factor (VEGF) gene
siRNA concentration 50 uM
Cell or Organism used HeLa cells
Transfection method Lipofectamine 2000
Duration after transfection 48 Hours
Article title Palmitic acid-conjugated 21-nucleotide siRNA enhances gene-silencing activity
Reference21985606