| Field | Sense | Antisense |
| siRNA chemical modification | 5-Amino* 2-Deoxythymidine | 5-Amino* 2-Deoxythymidine |
| siRNA modification types | 2 | 2 |
| Overall number of modifications | 3 | 3 |
| Position of modifications | 1 * 20,21 | 1 * 20,21 |
| Modification on sugar or base or phosphate | Phosphate; Sugar | Phosphate; Sugar |
| SMILES (Click to view structure & nomenclature) |
NCCCP(O)(=O)OCC1OCC(C1O)O CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O |
NCCCP(O)(=O)OCC1OCC(C1O)O CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O |
| siRNA Sequence | GCAGCACGACUUCUUCAAGTT | CUUGAAGAAGUCGUGCUGCTT |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | 5-N3ss/5-N3as | 5-N3ss/5-N3as |
| Biological activity | 18 percent target mRNA inhibition | |
| Experiment used to check activity | Dual Fluorescence Reporter Gene Assays | |
| Melting temperature (oC) | NA | |
| Target gene | GFP | |
| siRNA concentration | 50 nM | |
| Cell or Organism used | HeLa cells | |
| Transfection method | Lipofectamine | |
| Duration after transfection | 6 Hours | |
| Article title | RNAi in human cells: basic structural and functional features of small interfering RNA |
|
| Reference | 12408823 | |