SM4498 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 2-Deoxythymidine* Puromycin 2-Deoxythymidine* Puromycin
siRNA modification types 2 2
Overall number of modifications 3 3
Position of modifications 1 * 20,21 20,21 * 21
Modification on sugar or base or phosphate Phosphate; Sugar Sugar
SMILES
(Click to view structure & nomenclature)
CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O
COC1=CC=C(CC(N)C(=O)NC2C(CO)OC(C2O)N2C=NC3=C2N=CN=C3N(C)C)C=C1


OCC1OCCC1O
COC1=CC=C(CC(N)C(=O)NC2C(CO)OC(C2O)N2C=NC3=C2N=CN=C3N(C)C)C=C1


siRNA Sequence GCAGCACGACUUCUUCAAGTT CUUGAAGAAGUCGUGCUGCTT
siRNA length base-pair 21 21
siRNA name in paper ss3-Pmn/as3-Pmn ss3-Pmn/as3-Pmn
Biological activity 73 percent target mRNA inhibition
Experiment used to check activity Dual Fluorescence Reporter Gene Assays
Melting temperature (oC) NA
Target gene GFP
siRNA concentration 50 nM
Cell or Organism used HeLa cells
Transfection method Lipofectamine
Duration after transfection 42 Hours
Article title RNAi in human cells: basic structural and functional features of small interfering RNA
Reference12408823