| Field | Sense | Antisense |
| siRNA chemical modification | 2-Deoxythymidine | 2-Deoxythymidine* Biotin |
| siRNA modification types | 2 | 2 |
| Overall number of modifications | 3 | 3 |
| Position of modifications | 1 * 20,21 | 20,21 * 21 |
| Modification on sugar or base or phosphate | Phosphate; Sugar | Sugar |
| SMILES (Click to view structure & nomenclature) |
CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O |
OCC1OCCC1O OC(=O)CCCCC1SCC2NC(=O)NC12 |
| siRNA Sequence | GCAGCACGACUUCUUCAAGTT | CUUGAAGAAGUCGUGCUGCTT |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | ss/as3-Biotin | ss/as3-Biotin |
| Biological activity | 95 percent target mRNA inhibition | |
| Experiment used to check activity | Dual Fluorescence Reporter Gene Assays | |
| Melting temperature (oC) | NA | |
| Target gene | GFP | |
| siRNA concentration | 50 nM | |
| Cell or Organism used | HeLa cells | |
| Transfection method | Lipofectamine | |
| Duration after transfection | 42 Hours | |
| Article title | RNAi in human cells: basic structural and functional features of small interfering RNA |
|
| Reference | 12408823 | |