SM4502 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 2-Deoxythymidine 5-Amino* 2-Deoxythymidine
siRNA modification types 1 2
Overall number of modifications 2 3
Position of modifications 20,21 1 * 20,21
Modification on sugar or base or phosphate Sugar Phosphate; Sugar
SMILES
(Click to view structure & nomenclature)
CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O



NCCCP(O)(=O)OCC1OCC(C1O)O
CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O


siRNA Sequence GCAGCACGACUUCUUCAAGTT CUUGAAGAAGUCGUGCUGCTT
siRNA length base-pair 21 21
siRNA name in paper ss/5-N3as ss/5-N3as
Biological activity 17 percent target mRNA inhibition
Experiment used to check activity Dual Fluorescence Reporter Gene Assays
Melting temperature (oC) NA
Target gene GFP
siRNA concentration 50 nM
Cell or Organism used HeLa cells
Transfection method Lipofectamine
Duration after transfection 66 Hours
Article title RNAi in human cells: basic structural and functional features of small interfering RNA
Reference12408823