| Field | Sense | Antisense |
| siRNA chemical modification | 2-Deoxythymidine | 2-Deoxythymidine* Puromycin |
| siRNA modification types | 1 | 2 |
| Overall number of modifications | 2 | 3 |
| Position of modifications | 20,21 | 20,21 * 21 |
| Modification on sugar or base or phosphate | Sugar | Sugar |
| SMILES (Click to view structure & nomenclature) |
CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O |
OCC1OCCC1O COC1=CC=C(CC(N)C(=O)NC2C(CO)OC(C2O)N2C=NC3=C2N=CN=C3N(C)C)C=C1 |
| siRNA Sequence | GCAGCACGACUUCUUCAAGTT | CUUGAAGAAGUCGUGCUGCTT |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | ss/as3-Pmn | ss/as3-Pmn |
| Biological activity | 62 percent target mRNA inhibition | |
| Experiment used to check activity | Dual Fluorescence Reporter Gene Assays | |
| Melting temperature (oC) | NA | |
| Target gene | GFP | |
| siRNA concentration | 50 nM | |
| Cell or Organism used | HeLa cells | |
| Transfection method | Lipofectamine | |
| Duration after transfection | 66 Hours | |
| Article title | RNAi in human cells: basic structural and functional features of small interfering RNA |
|
| Reference | 12408823 | |