Field | Sense | Antisense |
siRNA chemical modification | 2-Deoxythymidine | 2-Deoxythymidine* Biotin |
siRNA modification types | 2 | 2 |
Overall number of modifications | 3 | 3 |
Position of modifications | 1 * 20,21 | 20,21 * 21 |
Modification on sugar or base or phosphate | Phosphate; Sugar | Sugar |
SMILES (Click to view structure & nomenclature) |
CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O |
OCC1OCCC1O OC(=O)CCCCC1SCC2NC(=O)NC12 |
siRNA Sequence | GCAGCACGACUUCUUCAAGTT | CUUGAAGAAGUCGUGCUGCTT |
siRNA length base-pair | 21 | 21 |
siRNA name in paper | ss/as3-Biotin | ss/as3-Biotin |
Biological activity | 86 percent target mRNA inhibition | |
Experiment used to check activity | Dual Fluorescence Reporter Gene Assays | |
Melting temperature (oC) | NA | |
Target gene | GFP | |
siRNA concentration | 50 nM | |
Cell or Organism used | HeLa cells | |
Transfection method | Lipofectamine | |
Duration after transfection | 66 Hours | |
Article title | RNAi in human cells: basic structural and functional features of small interfering RNA |
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Reference | 12408823 |