| Field | Sense | Antisense |
| siRNA chemical modification | Cyclohexenyl nucleic acid* 2-Deoxythymidine | 2-Deoxythymidine |
| siRNA modification types | 2 | 1 |
| Overall number of modifications | 4 | 2 |
| Position of modifications | 1,3 * 20,21 | 20,21 |
| Modification on sugar or base or phosphate | Sugar | Sugar |
| SMILES (Click to view structure & nomenclature) |
OCC1C=CCCC1OP(O)([O-])=O CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O |
OCC1OCCC1O |
| siRNA Sequence | GUAUUGACAGCUAUUCGAATT | UUCGAAUAGCUGUCAAUACTT |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | 2182 | siRNA-as(ORF1) |
| Biological activity | 73 percent target mRNA inhibition | |
| Experiment used to check activity | Immunostaining and Flow cytometry | |
| Melting temperature (oC) | NA | |
| Target gene | P-glycoprotein expression | |
| siRNA concentration | 50 nM | |
| Cell or Organism used | NIH 3T3-MDR cells | |
| Transfection method | Lipofectamine 2000 | |
| Duration after transfection | 72 Hours | |
| Article title | Structural characterization and biological evaluation of small interfering RNAs containing cyclohexenyl nucleosides |
|
| Reference | 17616127 | |