SM4518 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 2-Deoxythymidine Cyclohexenyl nucleic acid* 2-Deoxythymidine
siRNA modification types 1 2
Overall number of modifications 2 3
Position of modifications 20,21 18 * 20,21
Modification on sugar or base or phosphate Sugar Sugar
SMILES
(Click to view structure & nomenclature) 		CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O
OCC1C=CCCC1OP(O)([O-])=O
CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O
siRNA Sequence GUAUUGACAGCUAUUCGAATT UUCGAAUAGCUGUCAAUACTT
siRNA length base-pair 21 21
siRNA name in paper siRNA-ss 2185
Biological activity 77 percent target mRNA inhibition
Experiment used to check activity Immunostaining and Flow cytometry
Melting temperature (oC) NA
Target gene P-glycoprotein expression
siRNA concentration 50 nM
Cell or Organism used NIH 3T3-MDR cells
Transfection method Lipofectamine 2000
Duration after transfection 72 Hours
Article title Structural characterization and biological evaluation of small interfering RNAs containing cyclohexenyl nucleosides
Reference17616127