SM4529 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification Cyclohexenyl nucleic acid* 2-Deoxythymidine Cyclohexenyl nucleic acid* 2-Deoxythymidine
siRNA modification types 2 2
Overall number of modifications 3 3
Position of modifications 18 * 20,21 15 * 20,21
Modification on sugar or base or phosphate Sugar Sugar
SMILES
(Click to view structure & nomenclature) 		OCC1C=CCCC1OP(O)([O-])=O
CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O
OCC1C=CCCC1OP(O)([O-])=O
CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O
siRNA Sequence GAACUCUUAGCGUAUGCAATT UUGCAUACGCUAAGAGUUCTT
siRNA length base-pair 21 21
siRNA name in paper 2481 2483
Biological activity 36 percent target mRNA inhibition
Experiment used to check activity Immunostaining and Flow cytometry
Melting temperature (oC) NA
Target gene P-glycoprotein expression
siRNA concentration 50 nM
Cell or Organism used NIH 3T3-MDR cells
Transfection method Lipofectamine 2000
Duration after transfection 72 Hours
Article title Structural characterization and biological evaluation of small interfering RNAs containing cyclohexenyl nucleosides
Reference17616127