Field | Sense | Antisense |
siRNA chemical modification | 2-Deoxyribonucleotide | 0 |
siRNA modification types | 1 | 0 |
Overall number of modifications | 2 | 0 |
Position of modifications | 24,25 | 0 |
Modification on sugar or base or phosphate | Sugar | 0 |
SMILES (Click to view structure & nomenclature) |
OCC1OCCC1O |
- |
siRNA Sequence | GGCCUUUCACUACUCCUACGAGCAC | GUGCUCGUAGGAGUAGUGAAAGGCCAG |
siRNA length base-pair | 25 | 27 |
siRNA name in paper | DsiRNA-A | DsiRNA-A |
Biological activity | 70 percent target mRNA inhibition | |
Experiment used to check activity | Luciferase gene reporter assay | |
Melting temperature (oC) | NA | |
Target gene | Renilla luciferase gene | |
siRNA concentration | 0.2 nM | |
Cell or Organism used | HeLa cells | |
Transfection method | Lipofectamine 2000 | |
Duration after transfection | 48 Hours | |
Article title | Amino-modified and lipid-conjugated dicer-substrate siRNA enhances RNAi efficacy |
|
Reference | 22236254 |