SM4544 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 2-Deoxythymidine 2-Deoxythymidine
siRNA modification types 1 1
Overall number of modifications 2 2
Position of modifications 20,21 20,21
Modification on sugar or base or phosphate Sugar Sugar
SMILES
(Click to view structure & nomenclature)
CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O



CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O



siRNA Sequence CUUACGCUGAGUACUUCGATT UCGAAGUACUCAGCGUAAGTT
siRNA length base-pair 21 21
siRNA name in paper WT WT
Biological activity 83 percent target mRNA inhibition
Experiment used to check activity Luciferase gene reporter assay
Melting temperature (oC) 71.4
Target gene Firefly luciferase
siRNA concentration 0.008 nM
Cell or Organism used HeLa cells
Transfection method NA
Duration after transfection 24 Hours
Article title Efficient synthesis and cell-based silencing activity of siRNAS that contain triazole backbone linkages
Reference22260772