| Field | Sense | Antisense |
| siRNA chemical modification | 4-C- Aminomethyl-2-O-Methylribose | 0 |
| siRNA modification types | 1 | 0 |
| Overall number of modifications | 2 | 0 |
| Position of modifications | 17,20 | 0 |
| Modification on sugar or base or phosphate | Sugar | 0 |
| SMILES (Click to view structure & nomenclature) |
COC1COC(CN)(CO)C1O |
- |
| siRNA Sequence | GGCCUUUCACUACUCCUACUT | AGUAGGAGUAGUGAAAGGCCTT |
| siRNA length base-pair | 21 | 22 |
| siRNA name in paper | 12R-2R | 12R-2R |
| Biological activity | 98 percent target mRNA inhibition | |
| Experiment used to check activity | Dual luciferase reporter assay | |
| Melting temperature (oC) | 76.5 | |
| Target gene | Renilla luciferase | |
| siRNA concentration | 33 nM | |
| Cell or Organism used | HeLa cells | |
| Transfection method | Lipofectamine 2000 | |
| Duration after transfection | 48 Hours | |
| Article title | Synthesis, gene silencing, and molecular modeling studies of 4'-C-aminomethyl-2'-O-methyl modified small interfering RNAs |
|
| Reference | 22372696 | |