Field | Sense | Antisense |
siRNA chemical modification | 4-C- Aminomethyl-2-O-Methylribose | 4-C- Aminomethyl-2-O-Methylribose |
siRNA modification types | 1 | 1 |
Overall number of modifications | 2 | 2 |
Position of modifications | 5,7 | 3,21 |
Modification on sugar or base or phosphate | Sugar | Sugar |
SMILES (Click to view structure & nomenclature) |
COC1COC(CN)(CO)C1O |
COC1COC(CN)(CO)C1O |
siRNA Sequence | GGCCUUUCACUACUCCUACTT | AGUAGGAGUAGUGAAAGGCCUT |
siRNA length base-pair | 21 | 22 |
siRNA name in paper | 6R-7R | 6R-7R |
Biological activity | 55 percent target mRNA inhibition | |
Experiment used to check activity | Dual luciferase reporter assay | |
Melting temperature (oC) | NA | |
Target gene | Renilla luciferase | |
siRNA concentration | 33 nM | |
Cell or Organism used | HeLa cells | |
Transfection method | Lipofectamine 2000 | |
Duration after transfection | 48 Hours | |
Article title | Synthesis, gene silencing, and molecular modeling studies of 4'-C-aminomethyl-2'-O-methyl modified small interfering RNAs |
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Reference | 22372696 |