SM4621 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification Lauric acid 0
siRNA modification types 1 0
Overall number of modifications 1 0
Position of modifications 1 0
Modification on sugar or base or phosphate Phosphate 0
SMILES
(Click to view structure & nomenclature)
CCCCCCCCCCCC(=O)NCCCCCCO



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siRNA Sequence GGCCUUUCACUACUCCUACGA GUAGGAGUAGUGAAAGGCCAG
siRNA length base-pair 21 21
siRNA name in paper C12-si21Luc C12-si21Luc
Biological activity 85 percent target mRNA inhibition
Experiment used to check activity Dual-Glo Luciferase Assay System
Melting temperature (oC) NA
Target gene Renilla luciferase gene
siRNA concentration 1 nM
Cell or Organism used HeLa cells
Transfection method Lipofectamine 2000
Duration after transfection 48 Hours
Article title Lipid-conjugated 27-nucleotide double-stranded RNAs with dicer-substrate potency enhance RNAi-mediated gene silencing
Reference22494497