SM4630 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification Cholesterol 0
siRNA modification types 1 0
Overall number of modifications 1 0
Position of modifications 1 0
Modification on sugar or base or phosphate Phosphate 0
SMILES
(Click to view structure & nomenclature)
CC(C)CCCC(C)C1CCC2C3CC=C4CC(O)CCC4(C)C3CCC12C



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siRNA Sequence GGCCUUUCACUACUCCUACGA GUAGGAGUAGUGAAAGGCCAG
siRNA length base-pair 21 21
siRNA name in paper Chol-si21Luc Chol-si21Luc
Biological activity 67 percent target mRNA inhibition
Experiment used to check activity Dual-Glo Luciferase Assay System
Melting temperature (oC) NA
Target gene Renilla luciferase gene
siRNA concentration 0.2 nM
Cell or Organism used HeLa cells
Transfection method Lipofectamine 2000
Duration after transfection 48 Hours
Article title Lipid-conjugated 27-nucleotide double-stranded RNAs with dicer-substrate potency enhance RNAi-mediated gene silencing
Reference22494497