Field | Sense | Antisense |
siRNA chemical modification | Palmitic acid | 0 |
siRNA modification types | 1 | 0 |
Overall number of modifications | 1 | 0 |
Position of modifications | 1 | 0 |
Modification on sugar or base or phosphate | Phosphate | 0 |
SMILES (Click to view structure & nomenclature) |
CCCCCCCCCCCCCCCC(=O)NCCCCCCO |
- |
siRNA Sequence | CUGGCCUUUCACUACUCCUACGAGCAC | GUGCUCGUAGGAGUAGUGAAAGGCCAG |
siRNA length base-pair | 27 | 27 |
siRNA name in paper | C16-ds27Luc | C16-ds27Luc |
Biological activity | 58 percent target mRNA inhibition | |
Experiment used to check activity | Dual-Glo Luciferase Assay System | |
Melting temperature (oC) | NA | |
Target gene | Renilla luciferase gene | |
siRNA concentration | 600 nM | |
Cell or Organism used | HeLa cells | |
Transfection method | No transfection agent | |
Duration after transfection | 48 Hours | |
Article title | Lipid-conjugated 27-nucleotide double-stranded RNAs with dicer-substrate potency enhance RNAi-mediated gene silencing |
|
Reference | 22494497 |