Field | Sense | Antisense |
siRNA chemical modification | 0 | Locked nucleic acid* Thymidine |
siRNA modification types | 0 | 2 |
Overall number of modifications | 0 | 2 |
Position of modifications | 0 | 10 * 10 |
Modification on sugar or base or phosphate | 0 | Sugar ; Base |
SMILES (Click to view structure & nomenclature) |
- |
OCC12COC(CO1)C2O CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O |
siRNA Sequence | CUUACGCUGAGUACUUCGATT | UCGAAGUACUCAGCGUAAGTT |
siRNA length base-pair | 21 | 21 |
siRNA name in paper | 10 | 10 |
Biological activity | 91 percent target mRNA inhibition | |
Experiment used to check activity | Dual luciferase reporter assay | |
Melting temperature (oC) | NA | |
Target gene | Renilla luciferase | |
siRNA concentration | 13 nM | |
Cell or Organism used | HEK293 cells | |
Transfection method | Lipofectamine 2000 | |
Duration after transfection | NA | |
Article title | Modified Short Interfering Rna (Modified Sirna) |
|
Reference | US 20080249039 A1 |