| Field | Sense | Antisense |
| siRNA chemical modification | Locked nucleic acid | Locked nucleic acid |
| siRNA modification types | 1 | 1 |
| Overall number of modifications | 4 | 4 |
| Position of modifications | 1,2,10,14 | 11,12,19,20 |
| Modification on sugar or base or phosphate | Sugar | Sugar |
| SMILES (Click to view structure & nomenclature) |
OCC12COC(CO1)C2O |
OCC12COC(CO1)C2O |
| siRNA Sequence | NNNNNNNNNNNNNNNNNNNNNN | NNNNNNNNNNNNNNNNNNNNNN |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | J | J |
| Biological activity | 0 percent target mRNA inhibition | |
| Experiment used to check activity | Dual luciferase reporter assay | |
| Melting temperature (oC) | NA | |
| Target gene | Reverse orientation fLuc mRNA | |
| siRNA concentration | 30 nM | |
| Cell or Organism used | HeLa cells | |
| Transfection method | siPROT Amine transfection agent | |
| Duration after transfection | 48 Hours | |
| Article title | Sirna sequence-independent modification formats for reducing off-target phenotypic effects in rnai, and stabilized forms thereof |
|
| Reference | EP2548962A1 | |