| Field | Sense | Antisense |
| siRNA chemical modification | 5-Methylcytosine* Locked nucleic acid | Locked nucleic acid |
| siRNA modification types | 2 | 1 |
| Overall number of modifications | 3 | 3 |
| Position of modifications | 1 * 20,21 | 1,20,21 |
| Modification on sugar or base or phosphate | Sugar | Sugar |
| SMILES (Click to view structure & nomenclature) |
CC1=CN(C2OC(CO)C(O)C2O)C(=O)N=C1N OCC12COC(CO1)C2O |
OCC12COC(CO1)C2O |
| siRNA Sequence | CUUACGCUGAGUACUUCGATT | TCGAAGUACUCAGCGUAAGTT |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | 2185 | 2187 |
| Biological activity | 35 percent target mRNA inhibition | |
| Experiment used to check activity | Dual luciferase reporter assay | |
| Melting temperature (oC) | NA | |
| Target gene | Renilla luciferase | |
| siRNA concentration | 13 nM | |
| Cell or Organism used | HEK293 cells | |
| Transfection method | Lipofectamine 2000 | |
| Duration after transfection | 24 to 48 Hours | |
| Article title | Short interfering RNA (siRNA) analogues |
|
| Reference | US 8653252 B2 | |