SM4734 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification Locked nucleic acid Locked nucleic acid
siRNA modification types 1 1
Overall number of modifications 2 2
Position of modifications 20,21 20,21
Modification on sugar or base or phosphate Sugar Sugar
SMILES
(Click to view structure & nomenclature)
OCC12COC(CO1)C2O



OCC12COC(CO1)C2O



siRNA Sequence AUCUGAAGAAGGAGAAAAATT UUUUUCUCCUUCUUCAGAUTT
siRNA length base-pair 21 21
siRNA name in paper 2700-1 2699-1
Biological activity 93 percent target mRNA inhibition
Experiment used to check activity Dual luciferase reporter assay 
Melting temperature (oC) NA
Target gene Renilla luciferase
siRNA concentration 13 nM
Cell or Organism used HEK293 cells
Transfection method Lipofectamine 2000
Duration after transfection 24 to 48 Hours
Article title Short interfering RNA (siRNA) analogues 
Reference US 8653252 B2