Field | Sense | Antisense |
siRNA chemical modification | Locked nucleic acid | 2-Deoxy |
siRNA modification types | 1 | 1 |
Overall number of modifications | 3 | 2 |
Position of modifications | 1,20,21 | 20,21 |
Modification on sugar or base or phosphate | Sugar | Sugar |
SMILES (Click to view structure & nomenclature) |
OCC12COC(CO1)C2O |
OCC1OCCC1O |
siRNA Sequence | TGAGAGAAAGCACAGAAAATT | UUUUCUGUGCUUUCUCUCATT |
siRNA length base-pair | 21 | 21 |
siRNA name in paper | 2796 | NPY- |
Biological activity | 70 percent target mRNA inhibition | |
Experiment used to check activity | Real-time PCR | |
Melting temperature (oC) | NA | |
Target gene | NPY gene | |
siRNA concentration | 1 - 100 nM | |
Cell or Organism used | PC12 cells | |
Transfection method | Lipofectamine 2000 | |
Duration after transfection | 24 to 48 Hours | |
Article title | Short interfering RNA (siRNA) analogues |
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Reference | US 8653252 B2 |