| Field | Sense | Antisense |
| siRNA chemical modification | 4-Thioribose* Phosphorothioate | 2-O-Methylribose |
| siRNA modification types | 2 | 1 |
| Overall number of modifications | 36 | 7 |
| Position of modifications | 1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18 * 1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18 | 5,8,11,14,17,18,19 |
| Modification on sugar or base or phosphate | Sugar; Phosphate-linkage | Sugar |
| SMILES (Click to view structure & nomenclature) |
OCC1SCC(O)C1O OCC1OCC(O)C1OP(O)(S)=O |
COC1C(O)OC(CO)C1O |
| siRNA Sequence | GGAGAUCAACAUUUUCAAA | UUUGAAAAUGUUGAUCUCC |
| siRNA length base-pair | 19 | 19 |
| siRNA name in paper | 346287 | 352505 |
| Biological activity | IC-50 = 0.96445 nM for target mRNA | |
| Experiment used to check activity | RT-PCR | |
| Melting temperature (oC) | NA | |
| Target gene | Survivin | |
| siRNA concentration | 50 nM | |
| Cell or Organism used | HeLa cells | |
| Transfection method | LIPOFECTIN | |
| Duration after transfection | 16 Hours | |
| Article title | Positionally modified siRNA constructs |
|
| Reference | US 8394947 B2 | |