| Field | Sense | Antisense |
| siRNA chemical modification | 2-O-Methylribose | 2-O-Methylribose |
| siRNA modification types | 1 | 1 |
| Overall number of modifications | 18 | 7 |
| Position of modifications | 2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,20 | 6,7,10,11,18,19,20 |
| Modification on sugar or base or phosphate | Sugar | Sugar |
| SMILES (Click to view structure & nomenclature) |
COC1C(O)OC(CO)C1O |
COC1C(O)OC(CO)C1O |
| siRNA Sequence | AGAAGACACCUUCUGAGUAU | AUACUCAGAAGGUGUCUUCU |
| siRNA length base-pair | 20 | 20 |
| siRNA name in paper | 345857 | 345853 |
| Biological activity | IC-50 = 0.025677 nM for target mRNA | |
| Experiment used to check activity | RT-PCR | |
| Melting temperature (oC) | NA | |
| Target gene | eIF4E | |
| siRNA concentration | 50 nM | |
| Cell or Organism used | HeLa cells | |
| Transfection method | LIPOFECTIN | |
| Duration after transfection | 16 Hours | |
| Article title | Positionally modified siRNA constructs |
|
| Reference | US 8394947 B2 | |