SM4761 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 0 4-Thioribose* Phosphorothioate
siRNA modification types 0 2
Overall number of modifications 0 22
Position of modifications 0 1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19* 6,7,8
Modification on sugar or base or phosphate 0 Phosphate-linkage; Sugar
SMILES
(Click to view structure & nomenclature)
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OCC1SCC(O)C1O
OCC1OCC(O)C1OP(O)(S)=O


siRNA Sequence AAGUAAGGACCAGAGACAAA UUUGUCUCUGGUCCUUACUU
siRNA length base-pair 20 20
siRNA name in paper 308746 335226
Biological activity IC-50 = 1.57 nM for target mRNA
Experiment used to check activity RT-PCR
Melting temperature (oC) NA
Target gene PTEN
siRNA concentration 50 nM
Cell or Organism used HeLa cells
Transfection method LIPOFECTIN
Duration after transfection 16 Hours
Article title Positionally modified siRNA constructs
Reference US 8394947 B2