| Field | Sense | Antisense |
| siRNA chemical modification | 0 | 4-Thioribose* Phosphorothioate |
| siRNA modification types | 0 | 2 |
| Overall number of modifications | 0 | 22 |
| Position of modifications | 0 | 1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19* 6,7,8 |
| Modification on sugar or base or phosphate | 0 | Phosphate-linkage; Sugar |
| SMILES (Click to view structure & nomenclature) |
- |
OCC1SCC(O)C1O OCC1OCC(O)C1OP(O)(S)=O |
| siRNA Sequence | AAGUAAGGACCAGAGACAAA | UUUGUCUCUGGUCCUUACUU |
| siRNA length base-pair | 20 | 20 |
| siRNA name in paper | 308746 | 335226 |
| Biological activity | IC-50 = 1.57 nM for target mRNA | |
| Experiment used to check activity | RT-PCR | |
| Melting temperature (oC) | NA | |
| Target gene | PTEN | |
| siRNA concentration | 50 nM | |
| Cell or Organism used | HeLa cells | |
| Transfection method | LIPOFECTIN | |
| Duration after transfection | 16 Hours | |
| Article title | Positionally modified siRNA constructs |
|
| Reference | US 8394947 B2 | |