Field | Sense | Antisense |
siRNA chemical modification | 0 | Locked nucleic acid* 2-O-Methylribose |
siRNA modification types | 0 | 2 |
Overall number of modifications | 0 | 7 |
Position of modifications | 0 | 5,8,11,14 * 17,18,19 |
Modification on sugar or base or phosphate | 0 | Sugar; Sugar |
SMILES (Click to view structure & nomenclature) |
- |
OCC12COC(CO1)C2O COC1C(O)OC(CO)C1O |
siRNA Sequence | GGAGAUCAACAUUUUCAAA | UUUGAAAAUGUUGAUCUCC |
siRNA length base-pair | 19 | 19 |
siRNA name in paper | 343868 | 355712 |
Biological activity | IC-50 = 0.022046 nM for target mRNA | |
Experiment used to check activity | RT-PCR | |
Melting temperature (oC) | NA | |
Target gene | Survivin | |
siRNA concentration | 50 nM | |
Cell or Organism used | HeLa cells | |
Transfection method | LIPOFECTIN | |
Duration after transfection | 16 Hours | |
Article title | Positionally modified siRNA constructs |
|
Reference | US 8394947 B2 |