SM4770 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 0 Phosphorothioate
siRNA modification types 0 1
Overall number of modifications 0 6
Position of modifications 0 3,9,12,17,18,19
Modification on sugar or base or phosphate 0 Phosphate-linkage
SMILES
(Click to view structure & nomenclature)
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OCC1OCC(O)C1OP(O)(S)=O



siRNA Sequence GGAGAUCAACAUUUUCAAA UUUGAAAAUGUUGAUCUCC
siRNA length base-pair 19 19
siRNA name in paper 343868 353538
Biological activity IC-50 = 0.46 nM for target mRNA
Experiment used to check activity RT-PCR
Melting temperature (oC) NA
Target gene Survivin
siRNA concentration 50 nM
Cell or Organism used HeLa cells
Transfection method LIPOFECTIN
Duration after transfection 16 Hours
Article title Positionally modified siRNA constructs
Reference US 8394947 B2