Field | Sense | Antisense |
siRNA chemical modification | 0 | Phosphorothioate* 2-O-methoxyethylribose |
siRNA modification types | 0 | 2 |
Overall number of modifications | 0 | 22 |
Position of modifications | 0 | 1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19* 3,4,5 |
Modification on sugar or base or phosphate | 0 | Phosphate-linkage; Sugar |
SMILES (Click to view structure & nomenclature) |
- |
OCC1OCC(O)C1OP(O)(S)=O COCCOC1CSC(CO)C1O |
siRNA Sequence | AAGUAAGGACCAGAGACAAA | UUTGTCUCUGGUCCUUACUU |
siRNA length base-pair | 20 | 20 |
siRNA name in paper | 308746 | 330919 |
Biological activity | IC-50 = 29.4 nM for target mRNA | |
Experiment used to check activity | RT-PCR | |
Melting temperature (oC) | NA | |
Target gene | PTEN | |
siRNA concentration | 50 nM | |
Cell or Organism used | HeLa cells | |
Transfection method | LIPOFECTIN | |
Duration after transfection | 16 Hours | |
Article title | Positionally modified siRNA constructs |
|
Reference | US 8394947 B2 |