Field | Sense | Antisense |
siRNA chemical modification | Phosphorothioate | Phosphorothioate |
siRNA modification types | 1 | 1 |
Overall number of modifications | 1 | 1 |
Position of modifications | 20 | 20 |
Modification on sugar or base or phosphate | Sugar | Sugar |
SMILES (Click to view structure & nomenclature) |
OCC1OCC(O)C1OP(O)(S)=O |
OCC1OCC(O)C1OP(O)(S)=O |
siRNA Sequence | GGAUCAUCUCAAGUCUUACTT | GUAAGACUUGAGAUGAUCCTT |
siRNA length base-pair | 21 | 21 |
siRNA name in paper | I | I |
Biological activity | IC-50 = 0.0095 nM for target mRNA | |
Experiment used to check activity | 4 parameter fit model | |
Melting temperature (oC) | 71.8 | |
Target gene | Target gene | |
siRNA concentration | 10 nM | |
Cell or Organism used | Hep3B cells or rat H.II.4.E cells | |
Transfection method | Lipofectamine RNAiMax | |
Duration after transfection | 24 Hoursrs | |
Article title | Modified rnai agents |
|
Reference | WO2014022739A3 |