| Field | Sense | Antisense |
| siRNA chemical modification | 2-Fluororibose* Xylo-O-methylribose* Phosphorothioate | 2-Fluororibose* Xylo-O-methylribose* Phosphorothioate |
| siRNA modification types | 3 | 3 |
| Overall number of modifications | 12 | 12 |
| Position of modifications | 4,5,7,8,9,10,15,16,17,19 * 14 * 20 | 4,5,7,8,9,10,15,16,17,19 * 14 * 20 |
| Modification on sugar or base or phosphate | Sugar | Sugar |
| SMILES (Click to view structure & nomenclature) |
OCC1OCC(F)C1O COC1CCOC1CO OCC1OCC(O)C1OP(O)(S)=O |
OCC1OCC(F)C1O COC1CCOC1CO OCC1OCC(O)C1OP(O)(S)=O |
| siRNA Sequence | GGAUCAUCUCAAGUCUUACTT | GUAAGACUUGAGAUGAUCCTT |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | III | III |
| Biological activity | IC-50 = 0.0076 nM for target mRNA | |
| Experiment used to check activity | 4 parameter fit model | |
| Melting temperature (oC) | 73.4 | |
| Target gene | Target gene | |
| siRNA concentration | 10 nM | |
| Cell or Organism used | Hep3B cells or rat H.II.4.E cells | |
| Transfection method | Lipofectamine RNAiMax | |
| Duration after transfection | 24 Hoursrs | |
| Article title | Modified rnai agents |
|
| Reference | WO2014022739A3 | |