Field | Sense | Antisense |
siRNA chemical modification | Thymidine | Thymidine |
siRNA modification types | 1 | 1 |
Overall number of modifications | 2 | 2 |
Position of modifications | 20,21 | 20,21 |
Modification on sugar or base or phosphate | Sugar | Sugar |
SMILES (Click to view structure & nomenclature) |
CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O |
CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O |
siRNA Sequence | GGACAUUCAGAACAAGAAATT | UUUCUUGUUCUGAAUGUCCTT |
siRNA length base-pair | 21 | 21 |
siRNA name in paper | siRNA | siRNA |
Biological activity | 80 percent target mRNA inhibition | |
Experiment used to check activity | qRT PCR | |
Melting temperature (oC) | NA | |
Target gene | ApoB gene | |
siRNA concentration | 5nM | |
Cell or Organism used | HepG2 cells | |
Transfection method | RNAiMAX | |
Duration after transfection | 24 Hoursrs | |
Article title | Improved specificity of gene silencing by siRNAs containing unlocked nucleobase analogs |
|
Reference | 21047800 |